Vergleich

EnzyFluo AMPK Phosphorylation Assay Kit

Hersteller BioAssay Systems
Kategorie
Typ Assay-Kit
Specific against other
Menge 100 Tests
ArtNr BAS-EAMPK-100
Targets PRKAA1
eClass 6.1 32161000
eClass 9.0 32161090
Lieferbar
Description
For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells. Method: FL360/450nm, 530/585nm. Samples: cells. Species: human, mouse, rat. Procedure: 3 hrs (hands-on time). Size: 100 tests.
Faqs

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview

Application

  • For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells.

Key Features

  • Sensitive. Can measure pAMPK modulation in as little as 500 cells/well.
  • Simple and convenient. No cell lysis necessary, cells can be cultured for several days.

Method

  • FL360/450nm, 530/585nm

Samples

  • Cells

Species

  • Human, mouse, rat

Size

  • 100 tests

Shelf Life

  • 6 months

More Details

  • The 5-AMP-activated protein kinase (AMPK) is a key sensor of intracellular energy balance. AMPK is activated in response to an increase in the AMP/ATP ratio which can be caused by a number of factors such as muscle contraction, starvation, or hypoxia. AMPK is a heterotrimeric protein complex comprising of ?- (63 kDa), ?- (38 kDa) and ?- (38 kDa) subunits. For each subunit, isoforms have been identified (?1, ?2, ?- 1, ?- 2, ?1, ?2, ?3) which theoretically allow the formation of 12 different proteins. The ?-subunit contains a serine/threonine kinase domain and the regulatory subunits contain binding sites for AMP and ATP and for glycogen. AMPK is activated by phosphorylation on Thr-172 within the catalytic domain. AMP binding results in a 2 to 5-fold increase in AMPK activity compared to the basal level. Binding of AMP to the ?-subunit causes allosteric activation of the kinase and induces a conformational change in the kinase domain that protects AMPK from dephosphorylation of Thr-172.

    BioAssay Systems cell-based ELISA measures phosphorylated AMPK in whole cells and normalizes the signal to the total protein content. The antibody recognizes both ?-subunits and, thus, can be used for cells from all tissues (human, mouse, rat). This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study AMPK regulation in short-term and long-term assays. In this assay, cells grown in 96-well plates are fixed and permeabilized in the wells. AMPK phosphorylation (pAMPK) is measured using a fluorescent ELISA followed by total protein measurement in each well.
Shipment
ICE
Storage
-20 C

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 Tests
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 25.04.2024 

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen