Comparison

EnzyFluo AMPK Phosphorylation Assay Kit

Item no. BAS-EAMPK-100
Manufacturer BioAssay Systems
Amount 100 Tests
Category
Type Assay-Kit
Specific against other
Citations Cao, J. et al (2020). 6PGD Upregulation is Associated with Chemo-and Immuno-Resistance of Renal Cell Carcinoma via AMPK Signaling-Dependent NADPH-Mediated Metabolic Reprograming. The American journal of the medical sciences, 360(3), 279-286. Assay: Phosphorylated AMPK in human renal carcinoma cells.

Chen, Hu, et al (2019). 6PGD inhibition sensitizes hepatocellular carcinoma to chemotherapy via AMPK activation and metabolic reprogramming. Biomedicine & Pharmacotherapy 111: 1353-1358. Assay: AMPK phosphorylation in human hepatic cell.

Tsuda, Yuichi, et al (2017). "Trypsin-Treated beta-Lactoglobulin Improves Glucose Tolerance in C57BL/6 Mice by Enhancing AMPK Activation and Glucose Uptake in Hepatocytes." Biological and Pharmaceutical Bulletin 40.11: 1917-1922. Assay: AMPK phosphorylation in human hepatocytes.


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ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Available
Description
For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells. Method: FL360/450nm, 530/585nm. Samples: cells. Species: human, mouse, rat. Procedure: 3 hrs (hands-on time). Size: 100 tests.
Faqs

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview

Application

  • For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells.

Key Features

  • Sensitive. Can measure pAMPK modulation in as little as 500 cells/well.
  • Simple and convenient. No cell lysis necessary, cells can be cultured for several days.

Method

  • FL360/450nm, 530/585nm

Samples

  • Cells

Species

  • Human, mouse, rat

Size

  • 100 tests

Shelf Life

  • 6 months

More Details

  • The 5-AMP-activated protein kinase (AMPK) is a key sensor of intracellular energy balance. AMPK is activated in response to an increase in the AMP/ATP ratio which can be caused by a number of factors such as muscle contraction, starvation, or hypoxia. AMPK is a heterotrimeric protein complex comprising of ?- (63 kDa), ?- (38 kDa) and ?- (38 kDa) subunits. For each subunit, isoforms have been identified (?1, ?2, ?- 1, ?- 2, ?1, ?2, ?3) which theoretically allow the formation of 12 different proteins. The ?-subunit contains a serine/threonine kinase domain and the regulatory subunits contain binding sites for AMP and ATP and for glycogen. AMPK is activated by phosphorylation on Thr-172 within the catalytic domain. AMP binding results in a 2 to 5-fold increase in AMPK activity compared to the basal level. Binding of AMP to the ?-subunit causes allosteric activation of the kinase and induces a conformational change in the kinase domain that protects AMPK from dephosphorylation of Thr-172.

    BioAssay Systems cell-based ELISA measures phosphorylated AMPK in whole cells and normalizes the signal to the total protein content. The antibody recognizes both ?-subunits and, thus, can be used for cells from all tissues (human, mouse, rat). This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study AMPK regulation in short-term and long-term assays. In this assay, cells grown in 96-well plates are fixed and permeabilized in the wells. AMPK phosphorylation (pAMPK) is measured using a fluorescent ELISA followed by total protein measurement in each well.
Shipment
ICE
Storage
-20 C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 Tests
Available: In stock
available

Delivery expected until 8/28/2025 

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