QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Kit

RT

For quantitative colorimetric determination of glucose-6-phosphate concentration and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µ L sample): 0.2 to 100 U/L for 15 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD460nm. Samples: Plasma, serum, tissue and culture media, etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.2 U/L.

Application: For quantitative colorimetric determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism.
Key Features: Fast and sensitive. Linear detection range (20 ?L sample): 0.2 to 100 U/L for 15 min reaction.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method: OD460nm
Samples: Plasma, serum, tissue and culture media, etc
Species: All
Size: 100 tests
Detection Limit: 0.2 U/L
Shelf Life: 6 months
More Details: GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. BioAssay Systems' non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.