Comparison

QuantiChrom Glucose-6-Phosphate Dehydrogenase Kit

Item no. BAS-DGPDH-100
Manufacturer BioAssay Systems
Amount 100 Tests
Category
Type Kit
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Available
Description
For Quantitative colorimetric kinetic determination of Glucose-6-Phosphate Dehydrogenase activity and evaluation of drug effects on its metabolism. Method: OD565nm. Samples: plasma, serum, tissue and culture media etc. Species: all. Procedure: 30 min. Size
Faqs

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Overview

Application

  • For quantitative colorimetric determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism.

Key Features

  • Fast and sensitive. Linear detection range (20 ?L sample): 0.2 to 100 U/L for 15 min reaction.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • OD460nm

Samples

  • Plasma, serum, tissue and culture media, etc

Species

  • All

Size

  • 100 tests

Detection Limit

  • 0.2 U/L

Shelf Life

  • 6 months

More Details

  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. BioAssay Systems' non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Shipment
RT
Storage
-20 C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 Tests
Available: In stock
available

Delivery expected until 8/28/2025 

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