Anti-RIPK1 Antibody

ArtNr	BOS-A00141-1
Hersteller	Boster
Menge	0.1 mg
Quantity options	0.1 mg 0.1 mg
Kategorie	Cell Biology Cell Status Apoptosis Immunohistochemistry Western Blot ELISA Immunofluorescence Immunostaining
Format	Liquid
Applikationen	WB, IF, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Alias	RIP,RIP1,RIP,Cell death protein RIP,RIP-1
Lieferbar
Manufacturer - Category	Primary Antibodies
Storage Conditions	RIPK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	75931 MW
Clonality	Polyclonal
Application Details	RIPK1 antibody can be used for detection of RIPK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Gene Name	RIPK1
Gene Full Name	Receptor (TNFRSF)-interacting serine-threonine kinase 1
Background	RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NFκ-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-κB activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.
Immunogen	RIPK1 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human RIPK1. The immunogen is located within amino acids 180 - 230 of RIPK1.
Contents	RIPK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Purification	RIPK1 Antibody is affinity chromatography purified via peptide column.
Concentration	1 mg/mL
Manufacturer - Research Category	Apoptosis
Protein Name	Receptor (TNFRSF)-interacting serine-threonine kinase 1
Protein Function	Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage (PubMed:11101870, PubMed:17389591, PubMed:19524512, PubMed:19524513). Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor (PubMed:11101870, PubMed:17389591, PubMed:19524512, PubMed:19524513). Phosphorylates DAB2IP at 'Ser-728' in a TNF- alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade (PubMed:17389591). Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules (PubMed:15258597). Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death (PubMed:15258597). RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex (PubMed:19524513, PubMed:19524512).
Subcellular Localization	Cytoplasm. Cell membrane.
Description	Boster Bio Anti-RIPK1 Antibody (Catalog # A00141-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

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