Anti-RIPK1 Antibody

ArtNr	BOS-A00141
Hersteller	Boster
Menge	0.1 mg
Quantity options	0.1 mg 0.1 mg
Kategorie	Cell Biology Cell Status Apoptosis Immunohistochemistry ELISA Immunostaining
Format	Liquid
Applikationen	ELISA, IHC-P
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Alias	RIP,RIP1,RIP,Cell death protein RIP,RIP-1
Lieferbar
Manufacturer - Category	Primary Antibodies
Storage Conditions	RIPK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Molecular Weight	75931 MW
Clonality	Polyclonal
Application Details	RIPK1 antibody can be used for detection of RIP1 by immunohistochemistry at 5 - 10 μg/mL. Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Gene Name	RIPK1
Gene Full Name	Receptor (TNFRSF)-interacting serine-threonine kinase 1
Background	RIP1 (Receptor Interacting Protein), also known as RIPK1, is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIP1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIP1 is important for signallling to NF-kappa-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIP1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-kappa-B activation. RIP1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.
Immunogen	RIPK1 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human RIP1. The immunogen is located within amino acids 600 - 650 of RIPK1.
Contents	RIPK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Purification	RIPK1 Antibody is affinity chromatography purified via peptide column.
Concentration	1 mg/mL
Manufacturer - Research Category	Apoptosis
Protein Name	Receptor (TNFRSF)-interacting serine-threonine kinase 1
Protein Function	Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage (PubMed:11101870, PubMed:17389591, PubMed:19524512, PubMed:19524513). Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor (PubMed:11101870, PubMed:17389591, PubMed:19524512, PubMed:19524513). Phosphorylates DAB2IP at 'Ser-728' in a TNF- alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade (PubMed:17389591). Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules (PubMed:15258597). Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death (PubMed:15258597). RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex (PubMed:19524513, PubMed:19524512).
Subcellular Localization	Cytoplasm. Cell membrane.
Description	Boster Bio Anti-RIPK1 Antibody (Catalog # A00141). Tested in ELISA, IHC-P applications. This antibody reacts with Human.
Tissue Specificity	Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
Predicted Reactivity	Mouse

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