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Anti-SMURF1 Antibody Picoband®

Anti-SMURF1 Antibody Picoband®

ArtNr	BOS-PB9892-iFluor647
Hersteller	Boster
Menge	100 ug
Quantity options	100 ug/vial 10 ug 100 ug/vial 100 ug/vial 100 ug/vial 100 ug 100 ug 100 ug 100 ug/vial 100 ug/vial 100 ug/vial 100 ug/vial 100 ug
Kategorie	Neuroscience Neuroinflammation Neural Mitochondrial Function Aging & Neurological Disorders Protein Aggregation, Clearance & Misfolding Antibodies Cell Biology Cell Status Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Immunocytochemistry Autophagy Primary Antibodies Immunochemistry
Typ	Antibody Polyclonal
Format	Lyophilized
Applikationen	WB, FC, IF, IHC, ICC
Specific against	Human (Homo sapiens), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Sensitivity	<20 pg/ml
Citations	1. Entrez Gene: SMURF1 SMAD specific E3 ubiquitin protein ligase 1. 2. Yamaguchi K, Ohara O, Ando A, Nagase T (Apr 2008). Smurf1 ly targets hPEM-2, a GEF for Cdc42, via a novel combination of protein interaction modules in the ubiquitin-proteasome pathway. Biol. Chem. 389 (4): 405–13. 3. Zhu H, Kavsak P, Abdollah S, Wrana JL, Thomsen GH (Sep 1999). A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Nature 400 (6745): 687–93.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	E3 ubiquitin-protein ligase SMURF1,hSMURF1,6.3.2.-,SMAD ubiquitination regulatory factor 1,SMAD-specific E3 ubiquitin-protein ligase 1,SMURF1,KIAA1625
Lieferbar
Manufacturer - Category	Primary Antibodies
Manufacturer - Conjugate / Tag	iFluor647
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	86 kDa
Calculated Molecular weight	86114 MW
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
Gene Name	SMURF1
Gene Full Name	E3 ubiquitin-protein ligase SMURF1
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	E3 ubiquitin-protein ligase SMURF1 is an enzyme that in humans is encoded by the SMURF1 gene. It is mapped to 7q22.1. This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen	A synthetic peptide corresponding to a sequence in the middle region of human SMURF1, identical to the related mouse sequence.
Contents	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Cell Biology, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin E3 Enzymes
Protein Name	E3 ubiquitin-protein ligase SMURF1
Protein Function	E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Plays a role in dendrite formation by melanocytes (PubMed:23999003).
Subcellular Localization	Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Description	Boster Bio Anti-SMURF1 Antibody Picoband® catalog # PB9892. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Expressed in melanocytes (PubMed:23999003).
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins.

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BOS-PB9892-iFluor647-datasheet.pdf

BOS-PB9892-iFluor647-safety-datasheet.pdf