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Human recombinant TGF beta 2 (Transforming growth factor beta 2) protein, AF Europäischer Partner

ArtNr BOS-PROTP61812-4
Hersteller Boster
Menge 100 ug
Quantity options 100 ug 20 ug 5 ug
Kategorie
Format Lyophilized
Applikationen Cell Culture
Specific against Human (Homo sapiens)
Konjugat/Tag HIS
Sequence MALDAAYCFRNVQDNCCLRPLYIDFKRDLGWKWIHEPKGYNANFCAGACPYLWSSDTQHSRVLSLYNTINPEASASPCCVSQDLEPLTILYYIGKTPKIEQLSNMIVKSCKCS with polyhistidine tag at the C-terminus.
Alias G-TSF,LDS4,TGFB2
Lieferbar
Manufacturer - Category
Recombinant Proteins
Manufacturer - Conjugate / Tag
His Tag (C-term)
Storage Conditions
Lyophilized protein should be stored at -20°C for 1 year.
Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%
BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Molecular Weight
The protein has a calculated MW of 13.66 kDa.
The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
Manufacturer - Format
The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. If you have any concerns or special requirements, please confirm with us.
Calculated Molecular weight
The protein has a calculated MW of 13.66 kDa.The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
Gene Name
TGFB2
Purification
>98% as determined by SDS-PAGE.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile 10 mM HCl to a
concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least
20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Description
Transforming Growth Factors beta 2 (TGFβ-2) is a 12.85 kDa member of the epidermal Growth Factors with 113 amino acid residues. TGFβ-2 is expressed from throughout the body. TGFβ-2 is a regulator of cell proliferation, differentiation, apoptosis, cell plasticity and migration, etc. TGF-β-2 also associates with various kinds of diseases, such as cancer and tissue fibrosis.
Bioactivity/Biological Activity
Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells.
The ED₅₀ for this effect is <0.2 ng/mL.
The specific activity of recombinant human TGF beta 2 is > 5 x 10⁶ IU/mg.
Endotoxin level
<0.01 EU per 1 μg of the protein by the LAL method.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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