Anti-TREM1 Antibody Picoband™

Primary Antibodies, Rabbit Polyclonal Antibodies

Boster Bio Anti-TREM1 Antibody Picoband™ catalog # A02135-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.

Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x10⁶ cells

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

E. coli-derived human TREM1 recombinant protein (Position: A21-R200).

No cross-reactivity with other proteins.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

26 kDa

Isoform 1: Cell membrane ; Single-pass type I membrane protein.