Comparison

Anti-TREM1 Antibody Picoband™

Manufacturer Boster
Category
Type Antibody Polyclonal
Specific against Human
Isotype IgG
Format Lyophilized
Applications WB, FC, IHC, ICC
Amount 100 ug/vial
Host Rabbit
Item no. BOS-A02135-1
eClass 6.1 32160702
eClass 9.0 32160702
Available
Manufacturers Product Category
Primary Antibodies, Rabbit Polyclonal Antibodies
Manufacturers Research Category
Immunology, Innate Immunity, Macrophage/Inflammation
Short Description
Boster Bio Anti-TREM1 Antibody Picoband™ catalog # A02135-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.
Description
Boster Bio Anti-TREM1 Antibody Picoband™ catalog # A02135-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.
Background
Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.
Gene Name
TREM1
Application Details
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Clonality
Polyclonal
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen
E. coli-derived human TREM1 recombinant protein (Position: A21-R200).
Purification
Immunogen affinity purified.
Cross-reactivity
No cross-reactivity with other proteins.
Storage
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Gene Full Name
triggering receptor expressed on myeloid cells 1
Molecular Weight
26 kDa
Protein Function
Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.
Subcellular Localization
Isoform 1: Cell membrane ; Single-pass type I membrane protein.
Tissue Specificity
Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in normal cells than malignant cells. Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Strongly expressed in acute inflammatory lesions caused by bacteria and fungi. Isoform 2 was detected in the lung, liver and mature monocytes.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug/vial
Available: In stock
available

Delivery expected until 5/30/2024 

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