Anti-Zap-70 (Ab-319) Antibody

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Predicted MW: 70kd

Western blotting: 1:500~1:1000

Immunohistochemistry: 1:50~1:100

ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions
Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448
Ku GM, et al. (2001) EMBO J; 20(3): 457-465
Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780
Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774
Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844
Adjali O, et al. (2005) J Clin Invest; 115(8): 2287-2295.

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1 mg/ml

Boster Bio Anti-Zap-70 (Ab-319) Antibody (Catalog # A00754). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.