Hersteller |
Boster
|
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
Mouse, Rat |
Isotype |
IgG |
Format |
Lyophilized |
Applikationen |
WB, IF, IHC |
Menge |
100 ug/vial |
Host |
Rabbit |
ArtNr |
BOS-PA1050 |
Targets |
MBP |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Manufacturers Research Category |
Tags & Cell Markers |
Short Description |
Boster Bio Anti-Myelin Basic Protein/MBP Antibody catalog # PA1050. Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat. |
Description |
Boster Bio Anti-Myelin Basic Protein/MBP Antibody catalog # PA1050. Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat. |
Background |
Myelin basic protein (MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis. |
Gene Name |
MBP |
Application Details |
Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat Immunofluorescence, 2μg/ml, Mouse, Rat |
Application Notes |
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Predicted Reactivity |
Hamster |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3. |
Immunogen |
A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein, identical to the related rat and mouse sequences. |
Purification |
Immunogen affinity purified. |
Cross-reactivity |
No cross-reactivity with other proteins |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene Full Name |
Myelin basic protein |
Molecular Weight |
33117 MW |
Protein Function |
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.. |
Subcellular Localization |
Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic side of myelin. |
Tissue Specificity |
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.. |
Protein Name |
Myelin basic protein(MBP) |
Sequence Similarities |
Belongs to the myelin basic protein family. |
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