4E-BP1 (Phospho-Thr45) Colorimetric Cell-Based ELISA Kit

Store at 4°C for up to 6 months.

EIF4EBP1

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

The 4E-BP1 (Phospho-Thr45) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor 4E-BP1 (Phospho-Thr45) protein expression profile in cells. The kit can be used for measuring the relative amounts of 4E-BP1 (Phospho-Thr45) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on 4E-BP1 (Phospho-Thr45).