Adiponectin (111-247aa, globular domain), Mouse, Recombinant, expressed in E.coli

Adiponectin/Acrp30 (247amino acids) is adipocyte complement-related protein of 30kDa and exclusively expressed in differentiated adipocytes. Adiponectin (Acrp30) is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and globular domain (gAcrp30). Adiponectin (Acrp30) expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30 (247aa) or gAcrp30 (globular domain) lowers serum glucose and free fatty acid level in mice. The globular domain of adiponectin/acrp30(amino acid residues, 111-247, gAcrp30) was overexpressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

1 mg/ml (determined by Bradford assay)

• Joachim Fruebis, et al(2001) PNAS 98(4) 2005-2010
• Das, K., et al(2001) Biochem. Biophys.Res.Commun. 280(4)1120-1129
• Yamauchi T., et al(2001) Nature Medicine 7(8) 941-946
• Berg AH, et al(2001) Nature Medicine 7(8) 947-952
• Yamauchi. T., et al(2002) Nature Medicine 8(11) 1288-1295

>, 95% by SDS - PAGE

MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITV YMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYA DNVNDSTFTG FLLYHDTN