ZFAND3, 1-227aa, Human, His tag, E.coli

ZFAND3, also known as AN1-type zinc finger protein 3, contains DNA-binding domain and has a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZFAND3 is a 251 amino acid protein containing two AN1-type zinc fingers and two UIM (ubiquitin-interacting motif) repeats. Conserved in animals and plants, the AN1-type zinc finger domain is often found in proteins that contain a ubiquitin-like domain, which suggests a role in the ubiquitination pathway. Recombinant human ZFAND3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.5 mg/ml (determined by Bradford assay)

• Klug A. et al. (1999) J. Mol. Biol. 293: 215-218.
• Laity J.H. et al. (2007) Curr. Opin. Struct. Biol. 11: 39-46.

>, 90% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MGSHMGDAGS ERSKAPSLPP RCPCGFWGSS KTMNLCSKCF ADFQKKQPDD DSAPSTSNSQ SDLFSEETTS DNNNTSITTP TLSPSQQPLP TELNVTSPSK EECGPCTDTA HVSLITPTKR SCGTDSQSEN EASPVKRPRL LENTERSEET SRSKQKSRRR CFQCQTKLEL VQQELGSCRC GYVFCMLHRL PEQHDCTFDH MGRGREEAIM KMVKLDRKVG RSCQRIGEGC S