Anti-XPB/ERCC3 Antibody Picoband®

Primary Antibodies

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

64406 MW

Western blot, 0.1-0.5?g/ml

ELISA, 0.1-0.5?g/ml

ERCC excision repair 3, TFIIH core complex helicase subunit

XPB (xeroderma pigmentosum type B) is an ATP-dependent DNA helicase in humans that is a part of the TFIIH transcription factor complex. This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.

Each vial contains 4 mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05 mg NaN₃.

Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.

Cancer, Cancer Metabolism, DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Nucleotide Metabolism, Pathways and Processes, Response To Hypoxia

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP- dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.

Boster Bio Anti-XPB/ERCC3 Antibody Picoband® catalog # A03103-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.