Clusterin, Mouse Anti Human

Clusterin antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ca. 1:2, 000 and immunohistochemistry analysis is 1:100ca.200. Recommended starting dilution for Western blot is 1:1, 000 and Immunohistochemistry is 1:100. Mouse IgG1 heavy chain and k light chain. Anti-human Clusterin mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with Recombinant human Clusterin amino acids 1-333 purified from E. coli. Clusterin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. Mouse Anti Human Monoclonal.