Goat anti-apolipoprotein A-IV (mouse) Antibody

Pep ELISA, IHC

Goat anti-apolipoprotein A-IV (mouse) Antibody
Principle Name: apolipoprotein A-IV (mouse)
Official Symbol: Apoa4
All Name and Symbols: Apoa4; apolipoprotein A-IV; Apoa-4
Accession Number(s): NP_031494.2
Peptide Sequence: QETERVKEEIKKELED, is from internal region
Non-Human GeneID(s): 11808 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments in Mouse and Rat Liver lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Immunohistochemistry: In paraffin embedded Mouse Colon shows staining of cells between the villi, consistent with the observations with the related EB07581. Recommended concentration: 2-4ug/ml.
Reference(s): Orso E, Moehle C, Boettcher A, Szakszon K, Werner T, Langmann T, Liebisch G, Buechler C, Ritter M, Kronenberg F, Dieplinger H, Bornstein SR, Stremmel W, Schmitz G. The satiety factor apolipoprotein A-IV modulates intestinal epithelial permeability through its interaction with alpha-catenin: implications for inflammatory bowel diseases. Horm Metab Res. 2007 Aug; 39(8):601-11.PMID: 17712726