Goat anti-MK5 / MAPKAPK5 Antibody

Goat anti-MK5 / MAPKAPK5 Antibody
Principle Name: MK5 / MAPKAPK5
Official Symbol: Mapkapk5
All Name and Symbols: MAPKAPK5; mitogen-activated protein kinase-activated protein kinase 5; MAPKAP-K5; MK-5; MK5; PRAK; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; p38-regulated/activated protein kinase
Accession Number(s): NP_003659.2; NP_620777.1
Peptide Sequence: STEALDNVLPSAQ, is from internal region
Human Gene ID(s): 8550
Non-Human GeneID(s): 17165 (mouse); 498183 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB Trf, IP, IHC
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemi
Immunohistochemistry: In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8ug/ml.
Reference(s): Yoshizuka N, Chen RM, Xu Z, Liao R, Hong L, Hu WY, Yu G, Han J, Chen L, Sun P. A novel function of p38-regulated/activated kinase in endothelial cell migration and tumor angiogenesis. Mol Cell Biol. 2012 Feb; 32(3):606-18.PMID: 22124154