|
Hersteller |
Cell Sciences |
|
Kategorie |
|
|
Typ |
Antibody |
|
Specific against |
Human |
|
Isotype |
IgG |
|
Applikationen |
WB |
|
Menge |
100 ug |
|
Host |
Rabbit |
|
ArtNr |
CS-CPA103 |
|
Eclass 6.1 |
32160702 |
|
Eclass 9.0 |
32160702 |
|
Lieferbar |
 |
| Applications |
Western Blot |
| Description |
Rabbit Anti-Human APAF1 Affinity Purified polyclonal antibody. During apoptosis, APAF1 binds to cytochrome c (APAF2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase 9, and activates caspase 9 (APAF3). Activated caspase 9 in turn cleaves and activates caspase 3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. APAF1 is ubiquitously expressed in human tissues. |
| Formulation |
Liquid in PBS, pH 7.2 + 30% glycerol + 0.05% BSA + 0.01% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only. |
| Specificity |
Detects a 130 kDa protein corresponding to the apparent molecular weight of APAF1 on SDS-PAGE immunoblots. |
| Immunogen |
Synthetic peptide surrounding amino acid 1161 of human APAF1 |
| Purification |
Biospecific affinity chromatography |
| Storage and Stability |
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles. |
| Cross-Reactivity |
Reacts with human, mouse, rat, cow, rabbit, hamster, guinea pig, and monkey. |
