| Description |
Cytochrome c (m.w. 12, 500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases. |
| Specificity |
Detects the 12.6 kDa cytochrome c from human, mouse and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls. |
| Cross-Reactivity |
Human, Mouse, Rat, Bovine, Chick, Dog, Fruit fly, Horse, Kangaroo, Monkey, Pig, Pigeon, Rabbit, Sheep, Snail, Tuna, Turkey, Yeast (S. cerevisiae), Zebrafish |
| Application Notes |
For Western Blot, use a working dilution of 0.5-4 ug/ml.For Immunoprecipitation, use a working dilution of 10-20 ug/ml.Blocking Peptide (Cat. # CPC131BP) is available separately.The optimal concentration should be determined by the user for each specific application. |
