Antibodies

Rabbit Anti-Rat Cytochrome C Affinity Purified pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Rat
Isotype IgG
Applikationen WB
Menge 100 ug
Host Rabbit
ArtNr CS-CPC131
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot Immunoprecipitation
Description
Cytochrome c (m.w. 12, 500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
Formulation
Liquid in PBS, pH 7.2 + 30% glycerol + 0.5% BSA + 0.01% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
Detects the 12.6 kDa cytochrome c from human, mouse and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.
Immunogen
Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c
Purification
Biospecific affinity chromatography
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Concentration
0.2 mg/ml
Cross-Reactivity
Human, Mouse, Rat, Bovine, Chick, Dog, Fruit fly, Horse, Kangaroo, Monkey, Pig, Pigeon, Rabbit, Sheep, Snail, Tuna, Turkey, Yeast (S. cerevisiae), Zebrafish
Application Notes
For Western Blot, use a working dilution of 0.5-4 ug/ml.For Immunoprecipitation, use a working dilution of 10-20 ug/ml.Blocking Peptide (Cat. # CPC131BP) is available separately.The optimal concentration should be determined by the user for each specific application.

Arbeitsanleitung

CS-CPC131.pdf

Menge: 100 ug
Lieferbar: In stock
Listenpreis: 901,84 €
Preis: 901,84 €
lieferbar

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Arne Pelz

sales@hoelzel.de