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Rabbit Anti-Rat Cytochrome C Affinity Purified pAb

ArtNr CS-CPC131
Hersteller Cell Sciences
Menge 100 ug
Kategorie
Typ Antibody Polyclonal
Applikationen WB
Specific against Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products CYCSA
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Storage Conditions
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Application Notes
For Western Blot, use a working dilution of 0.5-4 ug/ml.For Immunoprecipitation, use a working dilution of 10-20 ug/ml.Blocking Peptide (Cat. # CPC131BP) is available separately.The optimal concentration should be determined by the user for each specific application.
Cross-Reactivity
Human, Mouse, Rat, Bovine, Chick, Dog, Fruit fly, Horse, Kangaroo, Monkey, Pig, Pigeon, Rabbit, Sheep, Snail, Tuna, Turkey, Yeast (S. cerevisiae), Zebrafish
Description
Cytochrome c (m.w. 12, 500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
Formulation
Liquid in PBS, pH 7.2 + 30% glycerol + 0.5% BSA + 0.01% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only.
Immunogen
Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c
Purification
Biospecific affinity chromatography
Specificity
Detects the 12.6 kDa cytochrome c from human, mouse and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.
Concentration
0.2 mg/ml

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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