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Rabbit Anti-Rat Cytochrome C Affinity Purified pAb

Rabbit Anti-Rat Cytochrome C Affinity Purified pAb

Manufacturer	Cell Sciences
Category	Antibodies Cell Biology Cell Status Apoptosis Western Blot Primary Antibodies
Type	Antibody Polyclonal
Specific against	Rat
Isotype	IgG
Applications	WB
Amount	100 ug
Host	Rabbit
Item no.	CS-CPC131
eClass 6.1	32160702
eClass 9.0	32160702
Available
Application Notes	For Western Blot, use a working dilution of 0.5-4 ug/ml.For Immunoprecipitation, use a working dilution of 10-20 ug/ml.Blocking Peptide (Cat. # CPC131BP) is available separately.The optimal concentration should be determined by the user for each specific application.
Concentration	0.2 mg/ml
Cross-Reactivity	Human, Mouse, Rat, Bovine, Chick, Dog, Fruit fly, Horse, Kangaroo, Monkey, Pig, Pigeon, Rabbit, Sheep, Snail, Tuna, Turkey, Yeast (S. cerevisiae), Zebrafish
Description	Cytochrome c (m.w. 12, 500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
Formulation	Liquid in PBS, pH 7.2 + 30% glycerol + 0.5% BSA + 0.01% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only.
Immunogen	Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c
Purification	Biospecific affinity chromatography
Storage and Stability	Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Specificity	Detects the 12.6 kDa cytochrome c from human, mouse and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

CS-CPC131.pdf

CS-CPC131-datasheet.pdf

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Amount: 100 ug

available

Delivery expected until 5/23/2024