Comparison

Rabbit Anti-Rat Cytochrome C Affinity Purified pAb

Item no. CS-CPC131
Manufacturer Cell Sciences
Amount 100 ug
Category
Type Antibody Polyclonal
Applications WB
Specific against Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products CYCSA
Available
Storage Conditions
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Application Notes
For Western Blot, use a working dilution of 0.5-4 ug/ml.For Immunoprecipitation, use a working dilution of 10-20 ug/ml.Blocking Peptide (Cat. # CPC131BP) is available separately.The optimal concentration should be determined by the user for each specific application.
Cross-Reactivity
Human, Mouse, Rat, Bovine, Chick, Dog, Fruit fly, Horse, Kangaroo, Monkey, Pig, Pigeon, Rabbit, Sheep, Snail, Tuna, Turkey, Yeast (S. cerevisiae), Zebrafish
Description
Cytochrome c (m.w. 12, 500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
Formulation
Liquid in PBS, pH 7.2 + 30% glycerol + 0.5% BSA + 0.01% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only.
Immunogen
Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c
Purification
Biospecific affinity chromatography
Specificity
Detects the 12.6 kDa cytochrome c from human, mouse and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.
Concentration
0.2 mg/ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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