|
Hersteller |
Cell Sciences
|
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Human |
|
Menge |
20 ug |
|
Host |
E.coli |
|
ArtNr |
CS-CRB201B |
|
eClass 6.1 |
34160400 |
|
eClass 9.0 |
42020190 |
|
Lieferbar |
 |
| Description |
Human 4-1BB Receptor, also named TNFRSF9, is a single non-glycosylated polypeptide chain containing 166 amino acids. 4-1BB Receptor is a member of the TNF superfamily of receptors. It is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. |
| Formulation |
0.2 um sterile filtered and lyophilized in 10 mM PB, pH 8.0, + 150 mM NaCl. |
| Reconstitution |
Centrifuge vial prior to opening. First add sterile distilled water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions. |
| Storage and Stability |
Store lyophilized protein at -20C to -80C. Reconstituted protein is stable for 1 week at 2-4C. For long term storage, aliquot and store at -20C to -80C with a carrier protein (0.1% HSA or BSA) as a stabilizer. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles. |
| GeneID |
3604 |
| Source |
E. coli |
| Molecular Weight |
17.7 kDa |
| Purity |
>95.0% as determined by analysis by SDS-PAGE and HPLC. |
| Endotoxin Level |
< 1 EU/ug as determined by LAL method. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity is measured by its binding ability in a functional ELISA. When Recombinant Human 4-1BB Receptor is immobilized at 10 ng/ml, the concentration of Recombinant Human 4-1BB Ligand that produces 50% optimal binding response is found to be approximately 0.5-2.5 ng/ml. |
| AA Sequence |
ERTRSLQDPC SNCPAGTFCD NNRNQICSPC PPNSFSSAGG QRTCDICRQC KGVFRTRKEC SSTSNAECDC TPGFHCLGAG CSMCEQDCKQ GQELTKKGCK DCCFGTFNDQ KRGICRPWTN CSLDGKSVLV NGTKERDVVC GPSPADLSPG ASSVTPPAPA REPGHS |
