Recombinant Human HIF-1 alpha

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
Host E.coli
ArtNr CS-CRC143B
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Recombinant Human HIF-1 alpha is a single, non-glycosylated polypeptide chain containing 298 amino acids (aa 530-826). Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that responds to changes in available oxygen in the cellular environment, specifically, to decreases in oxygen, or hypoxia. It belongs to the PER-ARNT-SIM (PAS) subfamily of the basic-helix-loop-helix (bHLH) family of transcription factors and is a heterodimer composed of alpha and beta subunits. Under hypoxic conditions, HIF-1 alpha activates the transcription of more than 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, VEGF, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 alpha also plays a crucial role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.
Formulation
Sterile filtered liquid solution in 20 mM Tris-HCl, pH 7.5, +1 mM DTT.
Storage and Stability
Stable for 1 month at 2-4C. For longer storage, aliquot and freeze at -80C. Avoid repeated freeze-thaw cycles.
Concentration
1 mg/ml
GeneID
3091
Source
E. coli
Molecular Weight
Predicted molecular weight of 32.8 kDa. The recombinant protein migrates as a 40 kDa band on SDS-PAGE
Purity
> 95.0% as determined by RP-HPLC and SDS-PAGE analyses
Endotoxin Level
< 0.1 ng/ug of protein
AA Sequence
MEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT kDaRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN

Arbeitsanleitung

CS-CRC143B.pdf

Menge: 50 ug
Lieferbar: In stock
Listenpreis: 428,68 €
Preis: 428,68 €
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