Recombinant Human IGF-1

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 20 ug
Host E.coli
ArtNr CS-CRI500A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
The Insulin-like Growth factors (IGFs) are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue, in vitro . The liver predominantly produces IGFs, although a variety of tissues produce IGFs at distinctive times. IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions, generates mature IGFs. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. Recombinant Human Insulin-like Growth Factor 1 is a single non-glycosylated polypeptide chain containing 70 amino acids.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
The lyophilized protein is stable at 2-8C. Upon receipt, store desiccated at -20C. After reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.
GeneID
3479
Source
E. coli
Molecular Weight
ca.7.6 kDa
Purity
>95.0% by HPLC and SDS-PAGE
Specific Activity
>5 x 105 units/mg
Endotoxin Level
<1 EU/ug as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml.
AA Sequence
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

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Menge: 20 ug
Lieferbar: In stock
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