Recombinant Human PIM1 GST Active *di

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 5 ug
Host Insect
ArtNr CS-CRP107A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Recombinant full length human Pim1 containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells. The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from Moloney mouse leukemia virus induced T-cell lymphomas in mice, Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Accumulating data support that the expression of Pim1 is mediated through activation of the JAK/STAT pathway. Some of the substrates of Pim1 include p21 Cip1, nuclear mitotic appartus protein, PTP-U2S and Socs-1. Recently, Pim1 has been shown to enhance the activities of p100, c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis. Pim1 interacts with c-Myb via the DNA binding domain and regulates its transcriptional activity.
Formulation
Recombinant protein in storage buffer (50 mM Tris-HCl + 150 mM NaCl + 0.25 mM DTT + 0.1 mM EGTA + 0.1 mM EDTA + 0.1 mM PMSF + 25% glycerol, pH, 7.5).
Storage and Stability
Store product frozen at or below -70C. Stable for 1 year at -70C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
Concentration
0.1 mg/ml
Source
Sf9 insect cells
Purity
1 ug of Pim1 protein was subjected to SDS-PAGE and Coomassie blue staining. The scan of the gel showed >90% purity of the Pim1 product, and the band was at ca.62 kDa (Fig. 2)
Specific Activity
316 nmol/min/mg: 316 nmol phosphate incorporated into the S6K substrate peptide per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay). See QA/QC section for details.

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Menge: 5 ug
Lieferbar: In stock
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