Recombinant Human PIM2 GST Active *di

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 10 ug
Host Insect
ArtNr CS-CRP108B
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Recombinant full-length human PIM 2 containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells. A 334-amino acid sequence of the clone was deduced, which showed 90% identity with the mouse Pim2 protein. Like mouse Pim2, the human protein appears to be a serine threonine kinase. Northern blot analysis detected 2 PIM2 transcripts in all tissues tested, but most abundantly in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It was also highly expressed in human leukemic and lymphoma cell lines and a colorectal adenocarcinoma cell line. The results suggested a role for PIM2 in proliferating cells as well as during meiosis. Potential functions were investigated for the pim family of kinases in factor-dependent murine hematopoietic cells and indicate that pim-2 functions similarly to pim-1 as a pro-survival kinase and suggest that BAD is a legitimate PIM-2 substrate. It was concluded that the transcriptional induction of Pim-2 initiated a novel NF-kappaB activation pathway that regulates cell survival.
Formulation
Recombinant protein in storage buffer (50 mM Tris-HCl + 150 mM NaCl + 0.25 mM DTT + 0.1 mM EGTA + 0.1 mM EDTA + 0.1 mM PMSF + 25% glycerol, pH, 7.5).
Storage and Stability
Store product frozen at or below -70C. Stable for 1 year at -70C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
Concentration
0.1 mg/ml
Source
Sf9 insect cells
Purity
2.5 ug of protein was subjected to SDS-PAGE and Coomassie blue staining. The scan of the gel showed >90% purity of the PIM 2 product, and the band was at ca.61 kDa (Fig. 2).
Specific Activity
305 nmol/min/mg: 305 nmol phosphate incorporated into the S6K substrate peptide per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay). See QA/QC section for details.

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Menge: 10 ug
Lieferbar: In stock
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