Recombinant Human RIPK2 GST Active *di

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 5 ug
Host Insect
ArtNr CS-CRR104A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Recombinant human RIPK2 (1-299) containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells. RIPK2 (RIP2, RICK) is a death domain-containing protein kinase. cDNAs were identified encoding a predicted 540-amino acid protein RICK , which contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. Which also demonstrated that RICK is a novel kinase that may regulate apoptosis induced by the FAS receptor pathway. Overexpression of RIP2 signaled both cell death and NF-kappa-B activation. RICK specifically interacted with the CARD of ICE/caspase-1, and this interaction correlated with the processing of procaspase- 1 and the formation of the active caspase-1 p20. Ripk2-deficient mice were generated and concluded that RIPK2 is implicated in the innate response to pathogens by NOD and TLR-induced cell signaling and mediates cytokine-induced Ifng production in Th1 and NK cells. It wa also demonstrated that RIPK2 is required for signaling through both TLR and NOD protein family members in the innate immune system as well as for appropriate TCR signaling in the adaptive immune response.
Formulation
Recombinant protein in storage buffer (50 mM Tris-HCl + 150 mM NaCl + 0.25 mM DTT + 0.1 mM EGTA + 0.1 mM EDTA + 0.1 mM PMSF + 25% glycerol, pH, 7.5).
Storage and Stability
Store product frozen at or below -70C. Stable for 1 year at -70C as undiluted stock. Aliquot to avoid repeated thawing and freezing.
Concentration
0.1 mg/ml
Source
Sf9 insect cells
Purity
1 ug of protein was subjected to SDS-PAGE and Coomassie blue staining. The scan of the gel showed >90% purity of the RIPK2 product, and the band was at ca.59 kDa (Fig. 2).
Specific Activity
20 nmol/min/mg: 20 nmol phosphate incorporated into MBP per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay). See QA/QC section for details.

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Menge: 5 ug
Lieferbar: In stock
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