Recombinant Human VEGFA (VEGF 165), Endotoxin Free_x0000_

Store lyophilized protein desiccated at -20C. Store reconstituted protein in working aliquots at -20C. Avoid repeated freeze-thaw cycles.

VEGF (Vascular endothelial growth factor) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculognesis and inhibits apoptosis. VEGF mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a promter of angiogenesis for human gliomas. Human VEGF occurs in several molecular variants and the 165 form is the most common form in most tissues. Biological activities of VEGF are not species-specific and glycosylation is not required for biological activity.

Centrifuge vial prior to opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 ug/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). The optimal concentration should be determined by the user for each specific application.

Bioactivity is assayed by measuring the dose dependent effect of recombinant human VEGF 165 on proliferation of Human uMbilical Vein Endothelial Cells (HUVEC) cells. This batch of growth factor was tested by an independent company and exhibits an ED50 ofor this effect is typically below 10 ng/ml/