Recombinant Mouse NGF beta

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Mouse
Menge 5 ug
Host E.coli
ArtNr CS-CRN006A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Nerve Growth Factor (NGF-beta) is a neurotrophic factor related to BDNF, NT-3 and NT-4. NGF-beta acts through its receptor beta-NGFR, and is involved in the development and maintenance of the sensory and sympathetic nervous systems. NGF-beta also is also involved in the growth, differentiation and survival of B lymphocytes. Human, mouse and rat proteins show cross-reactivity. Recombinant mouse NGF-beta is a non-glycosylated, non-covalently linked homodimer, containing two 120 amino acid chains.
Formulation
Recombinant mouse NGF-beta is lyophilized with no additives.
Reconstitution
Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Storage and Stability
Lyophilized product is very stable at -20C. Reconstituted material should be aliquoted and frozen at -20C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. Avoid repeated freeze-thaw cycles. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed.
GeneID
18049
Source
E. coli
Molecular Weight
13.5 kDa each
Purity
Typically 95% determined by: HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm
Specific Activity
5 x 105 Units/mg
Protein Accession No
Q6LDU8
Endotoxin Level
Measured by kinetic LAL analysis and is typically <= 1 EU/ug protein.
Biological Activity
The activity as determined by the proliferation of TF-1 cells and is typically less than 1 ng/mL.
AA Sequence
MSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G

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Menge: 5 ug
Lieferbar: In stock
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