Recombinant E. coli Thioredoxin

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against other
Menge 100 ug
Host E.coli
ArtNr CS-CRT138B
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. This could be especially useful in refolding proteins expressed in, E. coli, . To this end, thioredoxin has been shown to act as a protein disulfide isomerase.Its Molecular Weight is 11.9 kDa. and the pI is 4.67. Recombinant Thioredoxin was purified from, E. coli, harboring its gene.
Formulation
Each mg of protein contains 20 mM phosphate buffer pH 7.4.
Reconstitution
It is recommended to reconstitute the lyophilized TRX in sterile 18MU-cm water.
Storage and Stability
TRX although stable at 4C for 3 weeks, should be stored desiccated below -18C. Please prevent freeze thaw cycles.
GeneID
948289
Source
E. coli
Molecular Weight
11.9 kDa
Purity
Greater than 90.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Protein Accession No
POAA25
Biological Activity
TRX activity is assayed by measuring the change in absorbance at 650 nm at 25C using 0.13 uM bovine insulin containing 0.33 mM DTT (pH 6.5). The specific activity was found to be 3 IU/mg.
AA Sequence
HMSDKIIHLT DDSFDTDVLK ADGAILVDFW AEWCGPCKMI APILDEIGKL TVAKLNIDQN PGTAPKYGIR GIPTLLLFKN GEVAATKVGA LDANLA

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Menge: 100 ug
Lieferbar: In stock
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