Recombinant Protein A/G

Stable for 2 years at -20C. After reconstitution, aliquot and store at -20C. Avoid repeated freeze/thaw cycles.

The recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which correspond to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C2 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone. Specificity: The extended Fc-binding properties of Protein A/G make it a popular tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD, however, it does not bind mouse IgA, IgM or mouse serum albumin. Applications: Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone, occurring well at pH 5-8.

Dissolve in distilled water or saline.