Sarcoma 180 cells

Disease:

Rhabdomyosarcoma

Origin:

Derived from a malignant embryonal rhabdomyosarcoma from a 7 year old Caucasian female.

Species:

Homo sapiens

Tissue:

Muscle

Morphology:

Spindle cells and large multinucleated cells

Properties:

Adherent

Cytogenic data:

unstable within a hyperdiploid bimodal stemline number of 49 and 50. Twenty-two cells had chromosome associations, 15 cells microchromosomes, 2 cells had fragments, 2 cells with breaks, 2 cells with achromatic gaps and 1 cell with a secondary constriction.

Patient:

Female, 7 years of age, Caucasian

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

A subcultivation ratio of 1:2 to 1:4 is recommended. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO