Sarcoma 180 cells

Disease:
Rhabdomyosarcoma
Origin:
Derived from a malignant embryonal rhabdomyosarcoma from a 7 year old Caucasian female.
Species:
Homo sapiens
Tissue:
Muscle
Morphology:
Spindle cells and large multinucleated cells
Properties:
Adherent
Cytogenic data:
unstable within a hyperdiploid bimodal stemline number of 49 and 50. Twenty-two cells had chromosome associations, 15 cells microchromosomes, 2 cells had fragments, 2 cells with breaks, 2 cells with achromatic gaps and 1 cell with a secondary constriction.
Patient:
Female, 7 years of age, Caucasian
Medium:
AddexBio-formulated DMEM (C0003-01) + 10% FBS
Subculture:
A subcultivation ratio of 1:2 to 1:4 is recommended. Culture at 5% CO2, 37C.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 10, 11
D13S317: 13
D16S539: 10, 11
D5S818: 11
D7S820: 8, 12
THO1: 9.3
TPOX: 9
vWA: 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: RD (AddexBio C0035001)