RS-1

312756-74-4

C20H16Br2N2O3S

H2O : < 0.1 mg/mL (insoluble); DMSO : >= 100 mg/mL (190.76 mM)

Cell Cycle/DNA Damage; Cell Cycle/DNA Damage

RS-1 is a RAD51 activator, and also increases CRISPR/Cas9-mediated knock-in efficiencies. IC50 & Target: RAD51^[1], CRISPR/Cas9^[2] In Vitro: RS-1 is a RAD51 activator, stimulating binding of hRAD51 to DNA with K_d ranging from 48 nM to 107 nM in the presence of ATP or ADP and in the absence of a nucleotide cofactor, and such an effect is not via inhibiting its ATPase activity. RS-1 (20 uM) affects the length and helical pitch of hRAD51 protein-DNA complexes. RS-1 (0, 1, 5, 10, 15, 20, and 25 uM) stimulates strand assimilation activity of hRAD51. RS-1 (7.5 uM) promotes resistance of human cells to cross-linking chemotherapy^[1]. RS-1 (0, 7.5, 15 uM) increases Cas9-mediated knock-in efficiencies in rabbit embryos^[2].