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Phospho-PRKD1 (Tyr463) Antibody

ArtNr CSB-PA039755
Hersteller Cusabio
Menge 100 ul
Kategorie
Typ Antibody Polyclonal
Format Liquid
Applikationen WB, ELISA
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
Konjugat/Tag Unconjugated
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias KPCD1_HUMAN antibody; nPKC D1 antibody; nPKC mu antibody; nPKC-D1 antibody; nPKC-mu antibody; nPKCD1 antibody; nPKCmu antibody; PKC antibody; PKC MU antibody; PKCM antibody; PKCmu antibody; PKD 1 antibody; PKD antibody; PKD1 antibody; PRKCM antibody; PRKD 1 antibody; Prkd1 antibody; Protein kinase C mu antibody; Protein kinase C mu type antibody; Protein kinase D antibody; Protein kinase D1 antibody; Serine/threonine protein kinase D1 antibody; Serine/threonine-protein kinase D1 antibody
Similar products PRKD1, PKD, PKCM, KPCD1, PKC-mu
Lieferbar
Manufacturer - Type
Polyclonal Antibody
Manufacturer - Targets
PRKD1
Storage Conditions
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Manufacturer - Alias
KPCD1_HUMAN antibody; nPKC D1 antibody; nPKC mu antibody; nPKC-D1 antibody; nPKC-mu antibody; nPKCD1 antibody; nPKCmu antibody; PKC antibody; PKC MU antibody; PKCM antibody; PKCmu antibody; PKD 1 antibody; PKD antibody; PKD1 antibody; PRKCM antibody; PRKD 1 antibody; Prkd1 antibody; Protein kinase C mu antibody; Protein kinase C mu type antibody; Protein kinase D antibody; Protein kinase D1 antibody; Serine/threonine protein kinase D1 antibody; Serine/threonine-protein kinase D1 antibody
Immunogen Species
Human
Immunogen
Peptide sequence around phosphorylation site of Tyrosine463 (R-Y-Y(p)-K-E) derived from Human PKD1/PKC u.
Dilution
ELISA:1:2000-1:10000
WB:1:500-1:1000
Background

Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of MAPK8/JNK1 and Ras signaling, Golgi membrane integrity and trafficking, cell survival through NF-kappa-B activation, cell migration, cell differentiation by mediating HDAC7 nuclear export, cell proliferation via MAPK1/3 (ERK1/2) signaling, and plays a role in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced apoptosis and flagellin-stimulated inflammatory response. Phosphorylates the epidermal growth factor receptor (EGFR) on dual threonine residues, which leads to the suppression of epidermal growth factor (EGF)-induced MAPK8/JNK1 activation and subsequent JUN phosphorylation. Phosphorylates RIN1, inducing RIN1 binding to 14-3-3 proteins YWHAB, YWHAE and YWHAZ and increased competition with RAF1 for binding to GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). Acts downstream of the heterotrimeric G-protein beta/gamma-subunit complex to maintain the structural integrity of the Golgi membranes, and is required for protein transport along the secretory pathway. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane. May act by activating the lipid kinase phosphatidylinositol 4-kinase beta (PI4KB) at the TGN for the local synthesis of phosphorylated inositol lipids, which induces a sequential production of DAG, phosphatidic acid (PA) and lyso-PA (LPA) that are necessary for membrane fission and generation of specific transport carriers to the cell surface. Under oxidative stress, is phosphorylated at Tyr-463 via SRC-ABL1 and contributes to cell survival by activating IKK complex and subsequent nuclear translocation and activation of NFKB1. Involved in cell migration by regulating integrin alpha-5/beta-3 recycling and promoting its recruitment in newly forming focal adhesion. In osteoblast differentiation, mediates the bone morphogenic protein 2 (BMP2)-induced nuclear export of HDAC7, which results in the inhibition of HDAC7 transcriptional repression of RUNX2. In neurons, plays an important role in neuronal polarity by regulating the biogenesis of TGN-derived dendritic vesicles, and is involved in the maintenance of dendritic arborization and Golgi structure in hippocampal cells. May potentiate mitogenesis induced by the neuropeptide bombesin or vasopressin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. Plays an important role in the proliferative response induced by low calcium in keratinocytes, through sustained activation of MAPK1/3 (ERK1/2) pathway. Downstream of novel PKC signaling, plays a role in cardiac hypertrophy by phosphorylating HDAC5, which in turn triggers XPO1/CRM1-dependent nuclear export of HDAC5, MEF2A transcriptional activation and induction of downstream target genes that promote myocyte hypertrophy and pathological cardiac remodeling. Mediates cardiac troponin I (TNNI3) phosphorylation at the PKA sites, which results in reduced myofilament calcium sensitivity, and accelerated crossbridge cycling kinetics. The PRKD1-HDAC5 pathway is also involved in angiogenesis by mediating VEGFA-induced specific subset of gene expression, cell migration, and tube formation. In response to VEGFA, is necessary and required for HDAC7 phosphorylation which induces HDAC7 nuclear export and endothelial cell proliferation and migration. During apoptosis induced by cytarabine and other genotoxic agents, PRKD1 is cleaved by caspase-3 at Asp-378, resulting in activation of its kinase function and increased sensitivity of cells to the cytotoxic effects of genotoxic agents. In epithelial cells, is required for transducing flagellin-stimulated inflammatory responses by binding and phosphorylating TLR5, which contributes to MAPK14/p38 activation and production of inflammatory cytokines. May play a role in inflammatory response by mediating activation of NF-kappa-B. May be involved in pain transmission by directly modulating TRPV1 receptor.

D ppler H, Storz P (2007)J Biol Chem 282, 31873-81. Qin L, Zeng H, Zhao D (2006)J Biol Chem 281, 32550-8. Storz P, D ppler H, Toker A (2004)Mol Pharmacol 66, 870-9 .

Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugateds. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
General Research Areas
Signal Transduction
Initial Research Areas
Signal Transduction
Antigen Species
Human
Antigen
Peptide sequence around phosphorylation site of Tyrosine463 (R-Y-Y(p)-K-E) derived from Human PKD1/PKC μ .
Clonality
Polyclonal

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ul
Lieferbar: In stock
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