Hersteller |
Cusabio
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
Human |
Format |
Liquid or Lyophilized powder |
Menge |
1mg |
Host |
E.coli |
ArtNr |
CSB-EP016048HU-1 |
Konjugat/Tag |
Myc |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Research Areas |
Signal Transduction |
Target / Protein |
NR1I2 |
Biologically Active |
Not Test |
Expression System |
E.coli |
Species of origin |
Homo sapiens (Human) |
Uniprot ID |
O75469 |
AA Sequence |
MEVRPKESWNHADFVHCEDTESVPGKPSVNADEEV GGPQICRVCGDKATGYHFNVMTCEGCKGFFRRAMK RNARLRCPFRKGACEITRKTRRQCQACRLRKCLES GMKKEMIMSDEAVEERRALIKRKKSERTGTQPLGV QGLTEEQRMMIRELMDAQMKTFDTTFSHFKNFRLP GVLSSGCELPESLQAPSREEAAKWSQVRKDLCSLK VSLQLRGEDGSVWNYKPPADSGGKEIFSLLPHMAD MSTYMFKGIISFAKVISYFRDLPIEDQISLLKGAA FELCQLRFNTVFNAETGTWECGRLSYCLEDTAGGF QQLLLEPMLKFHYMLKKLQLHEEEYVLMQAISLFS PDRPGVLQHRVVDQLQEQFAITLKSYIECNRPQPA HRFLFLKIMAMLTELRSINAQHTQRLLRIQDIHPF ATPLMQELFGITGS |
Tag Info |
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged |
Expression Region |
1-434aa |
Protein Length |
Full Length |
MW |
69.8 kDa |
Distributor Discount |
50% off the list price |
Alternative Name(s) |
Orphan nuclear receptor PAR1 Orphan nuclear receptor PXR Pregnane X receptor Steroid and xenobiotic receptor |
Relevance |
Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes. |
Reference |
The human orphan nuclear receptor PXR is activated by compounds that regulate CYP3A4 gene expression and cause drug interactions. Lehmann J.M., McKee D.D., Watson M.A., Willson T.M., Moore J.T., Kliewer S.A. J. Clin. Invest. 102:1016-1023(1998) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes. |
Subcellular Location |
Nucleus |
Protein Families |
Nuclear hormone receptor family, NR1 subfamily |
Tissue Specificity |
Expressed in liver, colon and small intestine. |
Tag Information |
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged |
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