G-Actin : F-Actin In Vivo Assay Kit

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Hersteller Cytoskeleton
Typ Assay
Specific against other
Menge KIT 30-100 assays
ArtNr BK037
eClass 6.1 32161000
eClass 9.0 32161000
Delivery Time
1-2 Weeks
Shipping Temp.
Storage on Arrival

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Question 1:  At which step can the assay be stopped?

Answer 1:  The assay cannot be stopped until after the 100, 000 x g spin for 1 hour at 37°, C.  After this high speed centrifugation, the supernatant (G-actin) can be mixed with SDS loading buffer and frozen for later use.  The pellet (F-actin) should be resuspended with a depolymerizing agent and water and then mixed with SDS loading buffer and frozen for later use.  Upon freezing, F-actin depolymerizes, so it is necessary to separate the F-actin from the G-actin before freezing samples to isolate samples for an accurate measurement of F-actin and G-actin ratios.


Question 2:  How sensitive is this assay?

Answer 2:  The assay can detect as small as a 15% shift in G-actin to F-actin ratio.  Each condition should be performed in duplicate and repeated several times as assay reproducibility can vary by 10-20% between experiments. 



  If you have any questions concerning this product, please contact our Technical Service department at info@hoelzel.de

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Menge: KIT 30-100 assays
Lieferbar: In stock


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