Microtubule / Tubulin In Vivo Assay Kit

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Hersteller Cytoskeleton
Typ Assay
Specific against other
Menge KIT 30-100 assays
ArtNr BK038
eClass 6.1 32161000
eClass 9.0 32161000
Delivery Time
1-2 Weeks
Shipping Temp.
Storage on Arrival

Fan et al., 2012. A role for &gamma, S-crystallin in the organization of actin and fiber cell maturation in the mouse lens. FEBS J. v 279, pp 2892-2904.

Liu et al., 2011. A mechanism of Rap1-induced stabilization of endothelial cell&ndash, cell junctions. Mol. Biol. Cell. v 22, pp 2509-2519.

Mourad et al., 2011. Metabolic amplification of insulin secretion by glucose is independent of b-cell microtubules. Am. J. Physiol. Cell Physiol. v 300, pp C697&ndash, C706.

  Roth et al., 2007. A microtubule-facilitated nuclear import pathway for cancer regulatory proteins. Traffic. v 8, pp 673&ndash, 686.

Davis et al., 2005. Concurrent opposite effects of trichostatin A, an inhibitor of histone deacetylases, on expression of &alpha, -MHC and cardiac tubulins: implication for gain in cardiac muscle contractility. Am. J. Physiol. v 288, pp H1477-1490.



Question 1:  When lysing the cells, how do I prevent existing tubulin monomers from polymerizing onto existing microtubules?

Answer 1:  The microtubules/tubulin in vivo assay requires a constant cells-to-buffer volume ratio.  Essentially the lysis step has to dilute the cellular extract so that the free tubulin does not  polymerize onto existing microtubules (MTs). This ratio is roµ, ghly 10 volumes of buffer  to 1 volume of cell pellet.  Larger volumes of buffer are fine and in this kit the ratio is  targeted at 50 volumes of buffer per volume of cells.  Additionally, the average cell size is important in designing the experiment, so be sure to estimate the average cell size of your culture so that you can use it to calculate a good estimate for the volume of lysis buffer required.


Question 2:  Is it possible to quantify the absolute amount of total tubulin in each of the experimental samples?

Answer 2:  Absolute quantitation of cellular tubulin can be performed using the tubulin standard as a positive control at 50, 20, 10, 5 and 2 ng per lane.



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Menge: KIT 30-100 assays
Lieferbar: In stock


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