Rhodamine Phalloidin (14uM in methanol)

Hersteller Cytoskeleton
Kategorie
Typ Peptides
Specific against other
Menge 1 x 500 ul
ArtNr PHDR1
eClass 6.1 34160400
eClass 9.0 32160409
Lieferbar
Delivery Time
1-2 Weeks
Shipping Temp.
AT
Storage on Arrival
4C
Citations

Lee et al., 2013. Phellinus baumii extract influences pathogenesis of Brucella abortus in phagocyte by disrupting the phagocytic and intracellular trafficking pathway. J. Appl. Microbiol. 114, 329&ndash, 338.

Baruzzi and Berton, 2012. The tyrosine kinase Abl is a component of macrophage podosomes and is required for podosome formation and function. E. J. Immunol. doi: 10.1002/eji.201142270.

Shiozaki et al., 2012. XB130 as an Independent Prognostic Factor in Human Esophageal Squamous Cell Carcinoma. Ann. Surg. Oncology. doi: 10.1245/s10434-012-2474-4.

Gallo et al., 2012. Regulation of the Actin Cytoskeleton by Rho Kinase Controls Antigen Presentation by CD1d. J. Immunol. doi: 10.4049/Ÿ??jimmunol.1101484.

Lee et al., 2012. Dysadherin expression promotes the motility and survival of human breast cancer cells by AKT activation. Cancer Sci. v 103, pp 1280-1289.

Shi et al., 2012. MTA-enriched nanocomposite TiO2-polymeric powder coatings support human mesenchymal cell attachment and growth. Biomed. Mater. 7:055006. doi:10.1088/1748-6041/7/5/055006.

Ezratty et al., 2005. Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase. Nat. Cell Biol. v 7, pp 581-590.

Gomes et al., 2005. Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells. Cell. v 121, pp 451-463.

Chandhoke et al., 2004. &beta, 3 integrin phosphorylation is essential for Arp3 organization into leukocyte &alpha, V&beta, 3-vitronectin adhesion contacts. J. Cell Sci. v 117, pp 1431-1441.

Hsieh-Wilson et al., 2003. Phosphorylation of spinophilin modulates its interaction with actin filaments. J. Biol. Chem. v 278, pp 1186-1194.

Qian et al., 2002. PKC phosphorylation increases the ability of AFAP-110 to cross-link actin filaments. Mol. Biol. Cell. v 13, pp 2311-2322.

Chen, J., Fabry, B., Schiffrin, E. L. and Wang, N. (2001). Twisting integrin receptors increases endothelin-1 gene expression in endothelial cells. Am. J. Physiol. Cell Physiol. v 280, pp C1475-C1484.

faqs

Question 1:  Can I use fluorescently-labeled phalloidin to stain F-actin in living cells?

Answer 1:  Unfortunately, no, phalloidin cannot be used to stain F-actin in living cells.  Phalloidins are used to stain F-actin in fixed cells.  Fluorescent phalloidins only bind to the native quaternary structure of F-actin which provides a low background.  The correct fixation condition for phalloidin binding is 3.7% (v/v) paraformaldehyde in PBS  for 10 min because it retains the quaternary protein structure which is necessary for high affinity binding of phalloidin.  Methanol fixation destroys the native conformation and hence is not suitable for F-actin staining with phalloidin.  To monitor actin dynamics in living cells, micro-injection of rhodamine-labeled actin (Cat. # APHR or AR05) is recommended.  Please see those datasheets for more information.

 

Question 2:  Which of the fluorescently-labeled phalloidin is the most stable/brightest?

Answer 2:  The brightest and most stable of the Acti-stains is Acti-stain 488 (green fluorescence, Cat. # PHDG1).  Please see the table below for additional information on all of our Acti-stains. 

Conjugate

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Menge: 1 x 500 ul
Lieferbar: In stock
Listenpreis: 252,78 €
Preis: 252,78 €
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