Tubulin protein Soybean plant

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HiLyte Fluor 647labeled microtubules formed from HiLyte Fluor 647 labeled tubulin.

Porcine brain tubulin (>99% pure, see Cat. #T240) has been modified to contain covalently linked HiLyte Fluor 647 (HiLyte Fluor is a trademark of Anaspec Inc, CA) at random surface lysines. An activated ester of HiLyte Fluor 647 was used to label the protein. Labeling stoichiometry was determined by spectroscopic measurement of protein and dye concentrations (dye extinction coefficientWhen protein bound is 250, 000M^-1cm^-1). Final labeling stoichiometry is 0.2 to 0.7 dyes per tubulin heterodimer. HiLyte Fluor 647 labeled tubulin can be detected using a filter set of 600-630 nm excitation and 660-680 emission. HiLyte Fluor 647 tubulin is in a versatile, stable and easily shipped format. It is ready for micro-injection or in vitro polymerization. Cytoskeleton, Inc. also offers AMCA (Cat. # TL440M), HiLyte Fluor 488 (Cat. #TL488M), rhodamine (Cat. #TL590M), X-rhodamine (Cat. #TL620M) labeled tubulins.

Figure 1. HiLyte Fluor 647 tubulin protein purity determination. A 50 ug sample of unlabeled tubulin protein was separated by electrophoresis in a 4-20% SDS-PAGE system and stained with Coomassie Blue (A). Protein quantitation was performed using the Precision Red Protein Assay Reagent (Cat. # ADV02 ). 20 ug of the same protein sample was run in a 4-20% SDS-PAGE system and photographed directly under 525-625nm illumination (B).