ELISA Kit for Interleukin 10 (IL10)

7.8-500pg/mL

The minimum detectable dose of this kit is typically less than 2.8pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Interleukin 10 (IL10).
No significant cross-reactivity or interference between Interleukin 10 (IL10) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 (IL10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 (IL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 10 (IL10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa; Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease; Serum IL-6 and IL-10 concentrations in bitches with pyometra undergoing ovariohysterectomy; The immunomodulating effect of seminal plasma on T cells; Rituximab-induced HMGB1 release is associated with inhibition of STAT3 activity in human diffuse large B-cell lymphoma; Clinical significance of IL-2 and IL-10 gene polymorphisms and serum levels in patients with basal-cell carcinoma; Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period; Effects of acute exercise on salivary free insulin-like growth factor 1 and interleukin 10 in sportsmen.; Gemcitabine treatment enhanced the anti-tumor effect of cytokine induced killer cells by depletion of CD4+CD25bri regulatory T cells.; Clinical effect of electro-acupuncture on lung injury patients caused by severe; Commercial gold nanocolloid inhibits synthesis of IL-2 and proliferation of porcine T lymphocytes; The Level of Inflammatory Cytokines and Antimicrobial Peptides after Composite Material Implantation and Contamination with Bacterial Culture.; ВПЛИВ КРОВОВТРАТИ НА ДИНАМІКУ ВМІСТУ ЦИТОКІНІВ СИРОВАТКИ КРОВІ В ПЕРІОД ГОСТРОЇ РЕАКЦІЇ НА КРАНІОСКЕЛЕТНУ ТРАВМУ; Evidence-based Complementary and Alternative Medicine ; Attenuation of Bleomycin-induced pulmonary fibrosis in rats by flavocoxid treatment; Cytokine production in vitro and in rat model of colitis in response to Lactobacillus plantarum LS/07; Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancer; Association Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab.; Comparison of Plasma Interleukin-6 and Interleukin-10 Concentrations between Female Dogs with Mammary Gland Tumours and Healthy; Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease; Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signaling; Influence of Antibiotic-Impregnated Biomaterials on Inflammatory Cytokines; 1, 25-(OH) 2D3 protects Schwann cells against advanced glycation end products-induced apoptosis through PKA-NF-κB pathway; TRAF-6 调控TGF-β1-Smad2/Smad3 信号通路在Graves 病免疫发病机制中的作用; The impact of IL-10 gene polymorphism 1082A/G (rs1800896) on increased IL-10 secretion in patients with chronic kidney disease in the Kurdistan Region of …; Antibacterial immunomodulatory and antibiofilm triple effect of Salivaricin LHM against Pseudomonas aeruginosa urinary tract infection model;