« Back
Home /
ELISA Kit for Interleukin 10 (IL10)

ELISA Kit for Interleukin 10 (IL10)

Item no.	SEA056Hu-96T
Manufacturer	Cloud-Clone
Amount	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Neural Transcription Factors Targets for Rodent Neurosciences Aging & Neurological Disorders Infectious Diseases Viral Infections Coronavirus (COVID-19) Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Heart & Blood Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 2.8pg/mL
Citations	Serial cytokine levels during wound healing in rabbit maxillary sinus mucosaIncreased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary diseaseSerum IL-6 and IL-10 concentrations in bitches with pyometra undergoing ovariohysterectomyThe immunomodulating effect of seminal plasma on T cellsRituximab-induced HMGB1 release is associated with inhibition of STAT3 activity in human diffuse large B-cell lymphomaClinical significance of IL-2 and IL-10 gene polymorphisms and serum levels in patients with basal-cell carcinomaNeutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation periodEffects of acute exercise on salivary free insulin-like growth factor 1 and interleukin 10 in sportsmen.Gemcitabine treatment enhanced the anti-tumor effect of cytokine induced killer cells by depletion of CD4+CD25bri regulatory T cells.Clinical effect of electro-acupuncture on lung injury patients caused by severeCommercial gold nanocolloid inhibits synthesis of IL-2 and proliferation of porcine T lymphocytesThe Level of Inflammatory Cytokines and Antimicrobial Peptides after Composite Material Implantation and Contamination with Bacterial Culture.ВПЛИВ КРОВОВТРАТИ НА ДИНАМІКУ ВМІСТУ ЦИТОКІНІВ СИРОВАТКИ КРОВІ В ПЕРІОД ГОСТРОЇ РЕАКЦІЇ НА КРАНІОСКЕЛЕТНУ ТРАВМУEvidence-based Complementary and Alternative Medicine Attenuation of Bleomycin-induced pulmonary fibrosis in rats by flavocoxid treatmentCytokine production in vitro and in rat model of colitis in response to Lactobacillus plantarum LS/07Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancerAssociation Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab.Comparison of Plasma Interleukin-6 and Interleukin-10 Concentrations between Female Dogs with Mammary Gland Tumours and HealthyChanges in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary diseaseGrape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signalingInfluence of Antibiotic-Impregnated Biomaterials on Inflammatory Cytokines1, 25-(OH) 2D3 protects Schwann cells against advanced glycation end products-induced apoptosis through PKA-NF-κB pathwayTRAF-6 调控TGF-β1-Smad2/Smad3 信号通路在Graves 病免疫发病机制中的作用The impact of IL-10 gene polymorphism 1082A/G (rs1800896) on increased IL-10 secretion in patients with chronic kidney disease in the Kurdistan Region of …Antibacterial immunomodulatory and antibiofilm triple effect of Salivaricin LHM against Pseudomonas aeruginosa urinary tract infection modelCD38 play roles in T cell-dependent response and B cell differentiation in nile tilapia (Oreochromis niloticus)Altered expression of nuclear factor of activated T cells, Forkhead Box P3 and immune suppressive genes in regulatory T cells of generalized vitiligo patientsLevels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitisSerum sHLA-G: Significant diagnostic biomarker with respect to therapy and immunosuppressive mediators in Head and Neck Squamous Cell CarcinomaProgress in Neuropsychopharmacology & Biological PsychiatryClinical Importance of Neuregulin‐4 and Its Receptor ErbB4 in Periodontal Disease PathogenesisActive smoking, sleep quality and cerebrospinal fluid biomarkers of neuroinflammationDecreased suppression of CD8+ and CD4+ T cells by peripheral Regulatory T cells in Generalized Vitiligo due to reduced NFATC1 and FOXP3 proteinsCyclic Peptide Extracts Derived From Pseudostellaria heterophylla Ameliorates COPD via Regulation of the TLR4/MyD88 Pathway ProteinsIntraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairmentTanshinone IIA-loaded aligned microfibers facilitate stem cell recruitment and capillary formation by inducing M2 macrophage polarizationEffect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injuryAligned microfiber-induced macrophage polarization to guide schwann-cell-enabled peripheral nerve regenerationEffects of Systemic Lidocaine on Postoperative Recovery Quality and Immune Function in Patients Undergoing Laparoscopic Radical GastrectomyHOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED1Melatonin-mediated MT2 attenuates colitis induced by dextran sodium sulfate via PI3K/AKT/Nrf2/SIRT1/ROR¦Á/NF-¦ÊB signaling pathwaysInhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiotaGPNMB plays an active role in the M1/M2 balanceCytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control studyExpression of Antimicrobial Peptides and Cytokines in Human Omentum Following Abdominal SurgeryExploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization OutcomesInflammation promotes erythropoietin induced vascular calcification by activating p38 pathwayLung-Protective Effects of Lidocaine Infusion on Patients with Intermediate/High Risk of Postoperative Pulmonary Complications: A Double-Blind Randomized …Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot StudyCyclosporine A loaded brain targeting nanoparticle to treat cerebral ischemia/reperfusion injury in miceTranscutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel SyndromeAnalysis of IL-10 and IL-35 in DPP-4 inhibitor-related bullous pemphigoidEffectiveness of Carbonic Anhydrase Inhibitor Loaded Nanoparticles in the Treatment of Diabetic Retinopathy
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CSIF,IL10A,TGIF,Cytokine Synthesis Inhibitory Factor
Available
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Interleukin 10 (IL10). No significant cross-reactivity or interference between Interleukin 10 (IL10) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 (IL10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 (IL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 10 (IL10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Infection immunity;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.