sAPPalpha ELISA Kits (human, mouse, rat, rabbit)

Sodium Azide (0.1%)

Fully pre-configured sandwich ELISA format providing convenience, high sensitivity and selectivity.
Strip plate configuration allowing use and storage of separated sections of the ELISA plate.
Affinity purified polyclonal capture antibody on plate. Distinct HRP-conjugated. detection antibody.
Highly sensitive detection of sAPPalpha in cell culture medium and biological fluids. Detection ranges are 1-180 pg/ml depending on species. Detects the soluble alpha-secretase cleavage product of amyloid precursor protein in cell culture medium and biological fluids, designated sAPPalpha, after centrifugation and sedimentation of cells. Assays are done with the supernatant fraction to quantitate sAPPalpha.
This ELISA kit can also detect full length APP (amyloid precursor protein) in cell and tissue lysates after dissolution from the membrane. To distinguish sAPPalpha from membrane bound APP, first sediment cells and collect the supernatant to assay sAPPalpha. Then use a non-denaturing cell lysis buffer to release and solubilize full length APP from the cell pellet to assay the remaining uncleaved APP.
Rapid assay with colorimetric detection (measure reaction at 450nm). Total incubation with ELISA plate and HRP antibody is 1 hr at 37C or 2 hr at RT.