ArtNr |
Z02190-10 |
Hersteller |
GenScript
|
Menge |
10 ug |
Quantity options |
1 mg (2x500 ug)
10 ug
50 ug
|
Kategorie |
|
Typ |
Proteins |
Specific against |
Human (Homo sapiens) |
Purity |
The purity level of GenScript Recombinant Human GM-CSF is greater than 95.0%, as determined by the following methods: (a) RP-HPLC anaylsis (b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis |
Sequence |
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
protein/Z02190_10-GM_CSF_Sargramostim_Human,Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GenScript, Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) (Sargramostim) , is a single, glycosylated polypeptide chain containing 127 amino acids and having a molecular mass of about 26,000-32,000 Da, as shown in SDS-PAGE. It differs from natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein.</td></tr><tr><th>M.W.</th><td colspan="7"> 26,000-32,000 Da</td></tr><tr><th>Purity</th><td colspan="7"> The purity level of GenScript Recombinant Human GM-CSF is greater than 95.0%, as determined by the following methods: (a) RP-HPLC anaylsis (b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1 ng/ug (1 EU/ug) of sargramostim</td></tr><tr><th>Storage</th><td colspan="7"> Although lyophilized GenScript Sargramostim can remain stable at room temperature for three weeks, it is best stored desiccated below -18C. Upon reconstitution Sargramostim should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.</td></tr><tr><th>Specificity</th><td colspan="7"> The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.2 ng/ml, corresponding to a specific activity of 5.0×106 IU/ mg.</td></tr><tr><th>Formulation</th><td colspan="7"> The protein was lyophilized after extensive dialysis against 10mM Tris-HCl, pH8.5, 4% Mannitol, 1% Sucrose buffer.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended to reconstitute the lyophilized Sargramostim in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Sequence</th><td colspan="7"> The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.</td></tr> |
Similar products |
Granulocyte |
Lieferbar |
|
Specificity |
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.2 ng/ml, corresponding to a specific activity of 5.0x106 IU/ mg. |
Country of Origin |
USA |
Storage Conditions |
Although lyophilized GenScript Sargramostim can remain stable at room temperature for three weeks, it is best stored desiccated below -18C. Upon reconstitution Sargramostim should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
Molecular Weight |
26, 000-32, 000 Da |
Product Line |
Cytokine, Chemokines & Growth Factors |
Manufacturer - Specificity |
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.2 ng/ml, corresponding to a specific activity of 5.0x106 IU/ mg. |
Description |
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GenScript, Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) (Sargramostim) , is a single, glycosylated polypeptide chain containing 127 amino acids and having a molecular mass of about 26, 000-32, 000 Da, as shown in SDS-PAGE. It differs from natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein. |
Reconstitution |
It is recommended to reconstitute the lyophilized Sargramostim in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
Formulation |
The protein was lyophilized after extensive dialysis against 10mM Tris-HCl, pH8.5, 4% Mannitol, 1% Sucrose buffer. |
Endotoxin Level |
Less than 0.1 ng/ug (1 EU/ug) of sargramostim |
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