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Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Human (P. pastoris-expressed) Europäischer Partner

Hersteller GenScript
Kategorie
Typ Proteins
Specific against Human
Menge 50ug
ArtNr Z02190-50
Targets CD24
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Specificity
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.2 ng/ml, corresponding to a specific activity of 5.0x106 IU/ mg.
Description
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GenScript, Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) (Sargramostim) , is a single, glycosylated polypeptide chain containing 127 amino acids and having a molecular mass of about 26, 000-32, 000 Da, as shown in SDS-PAGE. It differs from natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein.
Endotoxin Level
Less than 0.1 ng/ug (1 EU/ug) of sargramostim
Formulation
The protein was lyophilized after extensive dialysis against 10mM Tris-HCl, pH8.5, 4% Mannitol, 1% Sucrose buffer.
M.W.
26, 000-32, 000 Da
Product Line
Cytokine, Chemokines & Growth Factors
Reconstitution
It is recommended to reconstitute the lyophilized Sargramostim in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Storage
Although lyophilized GenScript Sargramostim can remain stable at room temperature for three weeks, it is best stored desiccated below -18C. Upon reconstitution Sargramostim should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Product Origin
USA

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50ug
Lieferbar: In stock
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Lieferung vsl. bis 18.04.2024 

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