Thymic Stromal Lymphopoietin ( TSLP), Human

Hersteller GenScript
Kategorie
Typ Proteins
Specific against Human
Menge 10ug 
ArtNr Z02721-10
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Product Line
Cytokine, Chemokines & Growth Factors
Description
TSLP is a hemopoietic protein that is expressed in the heart, liver and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rª) and the TSLP-specific chain (TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP prohibited apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells (DCs). TSLP activated DCs primed naive T cells to produce the proallergic cytokines (IL-4, IL-5, IL-13, TNFª) while down-regulating IL-10 and IFN-I³ suggesting a role in initiating allergic inflammation.
M.W.
Approximately 15.0 KDa, a single non-glycosylated polypeptide chain containing 132 amino acids.
Purity
>98% by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 0.2EU/ug of rHuTSLP as determined by LAL method.
Specific Activity
Fully biologically active when compared to standard. The ED50 as measured by its ability to induce proliferation of human IL-7Rª and human TSLP R co-transfected mouse BaF3 cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3x106 units/mg.
Storage
This lyophilized preparation is stable at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles.
Formulation
Lyophilized from a 0.2µ, m filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °, C. Further dilutions should be made in appropriate buffered solutions.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Sequence
MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC L EQVSQLQG L WRRFNRPL LK QQ
Menge: 10ug 
Lieferbar: In stock
Listenpreis: 227,73 €
Preis: 227,73 €
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