IFN-γ, Mouse

ArtNr	Z02916-20
Hersteller	GenScript
Menge	20 ug
Quantity options	1 mg 100 ug 20 ug 500 ug
Kategorie	Oncology Neuroscience Neuroinflammation By Cell Type Carcinoma Hematology Myeloma Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines Cell Status Apoptosis Cardiovascular Research Ischemia & Reperfusion Injury Toxicity & Cytotoxicity Hypertension
Typ	Proteins
Specific against	Mouse (Murine, Mus musculus)
Purity	> 95% as analyzed by reducing SDS-PAGE.
Sequence	His23-Cys155 (accession number: P01580), expressed with an N-terminal Met.
Citations	Trinchieri, G. (2010) J Exp Med. 207:2053–63. Ealick, S.E. et al. (1991) Science. 252:698–702. Schroder, K. et al. (2004) J Leukoc Biol.75:163–89. Hu, X. et al. (2008) Immunol Rev. 226: 41–56. Wischhusen, J. et al. (2007) Semin Cancer Biol. 17:459–68.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02916_20-Interferon_gamma_IFN_Mouse, Sharing 41% sequence identity with human Interferon gamma (hIFN--gamma), mouse IFN gamma (mIFN--gamma), is a macrophage-activating factor.The active form of IFN--gamma, is an antiparallel dimer that sets off IFN--gamma/JAK/STAT pathway. IFN--gamma signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--gamma–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--gamma is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers. Recombinant, mouse IFN gamma (rmIFN-gamma), produced in, E. coli, is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--gamma has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.</td></tr><tr><th>M.W.</th><td colspan="7"> 15 kDa, observed by reducing SDS-PAGE.</td></tr><tr><th>Purity</th><td colspan="7"> > 95% as analyzed by reducing SDS-PAGE.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> <1 EU/ug, determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> ED50<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized recombinant, mouse IFN gamma (rmIFN-gamma), remains stable up to 6 months at -80C from date of receipt. Upon reconstitution, rmIFN-gamma should be stable up to 1week at 4C or up to 2 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized after extensive dialysis against PBS.</td></tr><tr><th>Reconstitution</th><td colspan="7"> Reconstituted in ddH2O or PBS at 100 ug/ml.</td></tr><tr><th>Sequence</th><td colspan="7"> His23-Cys155 (accession number: P01580), expressed with an N-terminal Met.</td></tr><tr><th>Reference</th><td colspan="7">Trinchieri, G. (2010) J Exp Med. 207:2053–63. Ealick, S.E. et al. (1991) Science. 252:698–702. Schroder, K. et al. (2004) J Leukoc Biol.75:163–89. Hu, X. et al. (2008) Immunol Rev. 226: 41–56. Wischhusen, J. et al. (2007) Semin Cancer Biol. 17:459–68.</td></tr>
Similar products	Interferon-gamma
Versandbedingung	Gekühlt
Lieferbar
Specificity	ED50<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	4°C
Storage Conditions	-80°C
Molecular Weight	15 kDa, observed by reducing SDS-PAGE.
Product Line	Cytokines
Manufacturer - Specificity	ED50<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.
Description	Sharing 41% sequence identity with human Interferon gamma (hIFN--gamma), mouse IFN gamma (mIFN--gamma), is a macrophage-activating factor.The active form of IFN--gamma, is an antiparallel dimer that sets off IFN--gamma/JAK/STAT pathway. IFN--gamma signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--gamma–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--gamma is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers. Recombinant, mouse IFN gamma (rmIFN-gamma), produced in, E. coli, is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--gamma has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Reconstitution	Reconstituted in ddH2O or PBS at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Endotoxin Level	<1 EU/ug, determined by LAL method.

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