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IFN-γ, Mouse

IFN-γ, Mouse

Item no.	Z02916-20
Manufacturer	GenScript
Amount	20 ug
Quantity options	1 mg 100 ug 20 ug 500 ug
Category	Oncology Neuroscience Neuroinflammation By Cell Type Carcinoma Hematology Myeloma Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines Cell Status Apoptosis Cardiovascular Research Ischemia & Reperfusion Injury Toxicity & Cytotoxicity Hypertension
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
Purity	> 95% as analyzed by reducing SDS-PAGE.
Sequence	His23-Cys155 (accession number: P01580), expressed with an N-terminal Met.
Citations	Trinchieri, G. (2010) J Exp Med. 207:2053–63. Ealick, S.E. et al. (1991) Science. 252:698–702. Schroder, K. et al. (2004) J Leukoc Biol.75:163–89. Hu, X. et al. (2008) Immunol Rev. 226: 41–56. Wischhusen, J. et al. (2007) Semin Cancer Biol. 17:459–68.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02916_20-Interferon_gamma_IFN_Mouse, Sharing 41% sequence identity with human Interferon gamma (hIFN--gamma), mouse IFN gamma (mIFN--gamma), is a macrophage-activating factor.The active form of IFN--gamma, is an antiparallel dimer that sets off IFN--gamma/JAK/STAT pathway. IFN--gamma signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--gamma–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--gamma is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers. Recombinant, mouse IFN gamma (rmIFN-gamma), produced in, E. coli, is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--gamma has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.</td></tr><tr><th>M.W.</th><td colspan="7"> 15 kDa, observed by reducing SDS-PAGE.</td></tr><tr><th>Purity</th><td colspan="7"> > 95% as analyzed by reducing SDS-PAGE.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> <1 EU/ug, determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> ED50<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized recombinant, mouse IFN gamma (rmIFN-gamma), remains stable up to 6 months at -80C from date of receipt. Upon reconstitution, rmIFN-gamma should be stable up to 1week at 4C or up to 2 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized after extensive dialysis against PBS.</td></tr><tr><th>Reconstitution</th><td colspan="7"> Reconstituted in ddH2O or PBS at 100 ug/ml.</td></tr><tr><th>Sequence</th><td colspan="7"> His23-Cys155 (accession number: P01580), expressed with an N-terminal Met.</td></tr><tr><th>Reference</th><td colspan="7">Trinchieri, G. (2010) J Exp Med. 207:2053–63. Ealick, S.E. et al. (1991) Science. 252:698–702. Schroder, K. et al. (2004) J Leukoc Biol.75:163–89. Hu, X. et al. (2008) Immunol Rev. 226: 41–56. Wischhusen, J. et al. (2007) Semin Cancer Biol. 17:459–68.</td></tr>
Similar products	Interferon-gamma
Shipping Condition	Cool pack
Available
Specificity	ED50<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	4°C
Storage Conditions	-80°C
Molecular Weight	15 kDa, observed by reducing SDS-PAGE.
Product Line	Cytokines
Manufacturer - Specificity	ED50<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.
Description	Sharing 41% sequence identity with human Interferon gamma (hIFN--gamma), mouse IFN gamma (mIFN--gamma), is a macrophage-activating factor.The active form of IFN--gamma, is an antiparallel dimer that sets off IFN--gamma/JAK/STAT pathway. IFN--gamma signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--gamma–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--gamma is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers. Recombinant, mouse IFN gamma (rmIFN-gamma), produced in, E. coli, is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--gamma has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Reconstitution	Reconstituted in ddH2O or PBS at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Endotoxin Level	<1 EU/ug, determined by LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Z02916-20-datasheet.pdf

Z02916-20-safety-datasheet.pdf