MOUSE ANTI HUMAN CYTOCHROME B245 HEAVY CHAIN

CYTOCHROME B245 HEAVY CHAIN

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Recognises the heavy chain of cytochrome B245 also known as gp91phox a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease characterised by severe recurrent infections. The heavy chain (gp91phox) along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b or Cyt b) which is always membrane-resident. Upon the appropriate stimulus other components of NADPH oxidase (p47phox p40phox p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. Clone NL7 binds amino acids 498-506 (EKDVITGLK) a cytosolic epitope on gp91phox not accessible on intact neutrophils. It recognises the native as well as the denatured form of the protein. Clone NL7 has an inhibitory effect on NADPH oxidase activation. Research indicates that this inhibitory effect is exerted during the initial stages of oxidase activation. The antibody does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane. Recommended Negative Controls: MOUSE IgG1 NEGATIVE CONTROLRecommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L)